Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms
Identifieur interne : 004036 ( Main/Exploration ); précédent : 004035; suivant : 004037Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms
Auteurs : L. F. Barros [Chili, Royaume-Uni] ; D. L. Yudilevich [Chili] ; S. M. Jarvis [Royaume-Uni] ; N. Beaumont [Royaume-Uni] ; J. D. Young [Canada] ; S. A. Baldwin [Royaume-Uni]Source :
- Pflügers Archiv [ 0031-6768 ] ; 1995-01-01.
Abstract
Abstract: Polyclonal antibodies raised against the human erythrocyte nucleoside transporter were used to investigate the distribution of the nucleoside transporters in the placenta. Immunoblots of brush-border membranes isolated from the human syncytiotrophoblast revealed a cross-reactive species that co-migrated with the erythrocyte nucleoside transporter as a broad band of apparent M r 55,000. In contrast, no labelling was detected in basal membranes containing a similar number of equilibrative nucleoside transporters as assessed by nitrobenzylthioinosine (NBMPR)-binding. The absence of cross-reactive epitopes in basal membranes and their presence in brush-border membranes was confirmed by confocal immunofluorescence microscopy. These results suggest that at least two isoforms of the NBMPR-sensitive nucleoside transporter are present in the human placenta. The lumenal surfaces of fetal capillaries, small placental vessels and umbilical vein were also strongly labelled by the antibody, a finding that suggests that the high fetal-placental adenosine uptake previously reported is due to endothelial transporters.
Url:
DOI: 10.1007/BF00374155
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002730
- to stream Istex, to step Curation: 002730
- to stream Istex, to step Checkpoint: 001A10
- to stream Main, to step Merge: 004758
- to stream Main, to step Curation: 004036
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms</title><author><name sortKey="Barros, L F" sort="Barros, L F" uniqKey="Barros L" first="L. F." last="Barros">L. F. Barros</name></author><author><name sortKey="Yudilevich, D L" sort="Yudilevich, D L" uniqKey="Yudilevich D" first="D. L." last="Yudilevich">D. L. Yudilevich</name></author><author><name sortKey="Jarvis, S M" sort="Jarvis, S M" uniqKey="Jarvis S" first="S. M." last="Jarvis">S. M. Jarvis</name></author><author><name sortKey="Beaumont, N" sort="Beaumont, N" uniqKey="Beaumont N" first="N." last="Beaumont">N. Beaumont</name></author><author><name sortKey="Young, J D" sort="Young, J D" uniqKey="Young J" first="J. D." last="Young">J. D. Young</name></author><author><name sortKey="Baldwin, S A" sort="Baldwin, S A" uniqKey="Baldwin S" first="S. A." last="Baldwin">S. A. Baldwin</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:34AB9EAB03B2FE77F019726BEB90FC803D4A258B</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1007/BF00374155</idno><idno type="url">https://api-v5.istex.fr/document/34AB9EAB03B2FE77F019726BEB90FC803D4A258B/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">002730</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002730</idno><idno type="wicri:Area/Istex/Curation">002730</idno><idno type="wicri:Area/Istex/Checkpoint">001A10</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001A10</idno><idno type="wicri:doubleKey">0031-6768:1995:Barros L:immunolocalisation:of:nucleoside</idno><idno type="wicri:Area/Main/Merge">004758</idno><idno type="wicri:Area/Main/Curation">004036</idno><idno type="wicri:Area/Main/Exploration">004036</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms</title><author><name sortKey="Barros, L F" sort="Barros, L F" uniqKey="Barros L" first="L. F." last="Barros">L. F. Barros</name><affiliation wicri:level="1"><country xml:lang="fr">Chili</country><wicri:regionArea>Departmento of Fisiología y Biofísica, Universidad de Chile, Casilla, 70005, Santiago 7</wicri:regionArea><wicri:noRegion>Santiago 7</wicri:noRegion></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Leeds, LS2 9JT, Leeds</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author><author><name sortKey="Yudilevich, D L" sort="Yudilevich, D L" uniqKey="Yudilevich D" first="D. L." last="Yudilevich">D. L. Yudilevich</name><affiliation wicri:level="1"><country xml:lang="fr">Chili</country><wicri:regionArea>Departmento of Fisiología y Biofísica, Universidad de Chile, Casilla, 70005, Santiago 7</wicri:regionArea><wicri:noRegion>Santiago 7</wicri:noRegion></affiliation></author><author><name sortKey="Jarvis, S M" sort="Jarvis, S M" uniqKey="Jarvis S" first="S. M." last="Jarvis">S. M. Jarvis</name><affiliation wicri:level="1"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Research School of Biosciences, University of Kent, CT2 7NJ, Canterbury, Kent</wicri:regionArea><wicri:noRegion>Kent</wicri:noRegion></affiliation></author><author><name sortKey="Beaumont, N" sort="Beaumont, N" uniqKey="Beaumont N" first="N." last="Beaumont">N. Beaumont</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Leeds, LS2 9JT, Leeds</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author><author><name sortKey="Young, J D" sort="Young, J D" uniqKey="Young J" first="J. D." last="Young">J. D. Young</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Physiology, University of Alberta, T6G 2H7, Edmonton</wicri:regionArea><wicri:noRegion>Edmonton</wicri:noRegion></affiliation></author><author><name sortKey="Baldwin, S A" sort="Baldwin, S A" uniqKey="Baldwin S" first="S. A." last="Baldwin">S. A. Baldwin</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biochemistry and Molecular Biology, University of Leeds, LS2 9JT, Leeds</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Pflügers Archiv</title><title level="j" type="sub">European Journal of Physiology</title><title level="j" type="abbrev">Pflugers Arch.</title><idno type="ISSN">0031-6768</idno><idno type="eISSN">1432-2013</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1995-01-01">1995-01-01</date><biblScope unit="volume">429</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="394">394</biblScope><biblScope unit="page" to="399">399</biblScope></imprint><idno type="ISSN">0031-6768</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0031-6768</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Polyclonal antibodies raised against the human erythrocyte nucleoside transporter were used to investigate the distribution of the nucleoside transporters in the placenta. Immunoblots of brush-border membranes isolated from the human syncytiotrophoblast revealed a cross-reactive species that co-migrated with the erythrocyte nucleoside transporter as a broad band of apparent M r 55,000. In contrast, no labelling was detected in basal membranes containing a similar number of equilibrative nucleoside transporters as assessed by nitrobenzylthioinosine (NBMPR)-binding. The absence of cross-reactive epitopes in basal membranes and their presence in brush-border membranes was confirmed by confocal immunofluorescence microscopy. These results suggest that at least two isoforms of the NBMPR-sensitive nucleoside transporter are present in the human placenta. The lumenal surfaces of fetal capillaries, small placental vessels and umbilical vein were also strongly labelled by the antibody, a finding that suggests that the high fetal-placental adenosine uptake previously reported is due to endothelial transporters.</div></front></TEI><affiliations><list><country><li>Canada</li><li>Chili</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Yorkshire-et-Humber</li></region><settlement><li>Leeds</li></settlement><orgName><li>Université de Leeds</li></orgName></list><tree><country name="Chili"><noRegion><name sortKey="Barros, L F" sort="Barros, L F" uniqKey="Barros L" first="L. F." last="Barros">L. F. Barros</name></noRegion><name sortKey="Yudilevich, D L" sort="Yudilevich, D L" uniqKey="Yudilevich D" first="D. L." last="Yudilevich">D. L. Yudilevich</name></country><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Barros, L F" sort="Barros, L F" uniqKey="Barros L" first="L. F." last="Barros">L. F. Barros</name></region><name sortKey="Baldwin, S A" sort="Baldwin, S A" uniqKey="Baldwin S" first="S. A." last="Baldwin">S. A. Baldwin</name><name sortKey="Beaumont, N" sort="Beaumont, N" uniqKey="Beaumont N" first="N." last="Beaumont">N. Beaumont</name><name sortKey="Jarvis, S M" sort="Jarvis, S M" uniqKey="Jarvis S" first="S. M." last="Jarvis">S. M. Jarvis</name></country><country name="Canada"><noRegion><name sortKey="Young, J D" sort="Young, J D" uniqKey="Young J" first="J. D." last="Young">J. D. Young</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004036 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004036 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:34AB9EAB03B2FE77F019726BEB90FC803D4A258B
|texte= Immunolocalisation of nucleoside transporters in human placental trophoblast and endothelial cells: evidence for multiple transporter isoforms
}}
| This area was generated with Dilib version V0.6.29. |  |